Application
Note #1:
Using large-volume
superconvective PCR to overcome PCR inhibition
Large-volume superconvective
real-time PCR*, using the QuanTyper?48
instrument, overcomes inhibitor induced
amplification failure in bovine feces samples.
This could have positive implications in
situations where diagnosis of a disease
is based on real-time PCR analysis of DNA
originating from bovine feces, e.g. Johne’s
disease.
Read
the complete text
|
|
Application
Note #2:
Using superconvective
DNA amplification to speed up Sanger sequence
analysis of forensic mitochondrial DNA samples
Quick and simple preparation/amplification
of DNA samples, combined with fast DNA sequencing,
is of great interest to the forensic genetics
community. In this study the QuanTyper?48
instrument has been used to speed up the
amplification steps involved in Sanger DNA
sequencing.
Read
the complete text
|
Application
Note #3
Discrimination of base-pair
neutral single nucleotide polymorphism (SNP)
by highresolution melting on QuanTyper?48
High-resolution melting
(HRM) analysis has recently emerged as a
simple, rapid and cost-effective method
for mutation screening and genotyping of
single nucleotide polymorphisms. In this
study, we show that the most subtle and
hard to detect genetic variation, an A/T
conversion, is possible to genotype by HRM
on QuanTyper-48.
Read
the complete text |
|
|
