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Applications | Application notes
 

Application Note #1:

Using large-volume superconvective PCR to overcome PCR inhibition

Large-volume superconvective real-time PCR*, using the QuanTyper™-48 instrument, overcomes inhibitor induced amplification failure in bovine feces samples. This could have positive implications in situations where diagnosis of a disease is based on real-time PCR analysis of DNA originating from bovine feces, e.g. Johne’s disease.
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Application Note #2:

Using superconvective DNA amplification to speed up Sanger sequence analysis of forensic mitochondrial DNA samples

Quick and simple preparation/amplification of DNA samples, combined with fast DNA sequencing, is of great interest to the forensic genetics community. In this study the QuanTyper™-48 instrument has been used to speed up the amplification steps involved in Sanger DNA sequencing.
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Application Note #3

Discrimination of base-pair neutral single nucleotide polymorphism (SNP) by highresolution melting on QuanTyper™-48

High-resolution melting (HRM) analysis has recently emerged as a simple, rapid and cost-effective method for mutation screening and genotyping of single nucleotide polymorphisms. In this study, we show that the most subtle and hard to detect genetic variation, an A/T conversion, is possible to genotype by HRM on QuanTyper-48.
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