Application
Note #1:
Using large-volume
superconvective PCR to overcome PCR inhibition
Large-volume superconvective
real-time PCR*, using the QuanTyper™-48
instrument, overcomes inhibitor induced
amplification failure in bovine feces samples.
This could have positive implications in
situations where diagnosis of a disease
is based on real-time PCR analysis of DNA
originating from bovine feces, e.g. Johne’s
disease.
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Application
Note #2:
Using superconvective
DNA amplification to speed up Sanger sequence
analysis of forensic mitochondrial DNA samples
Quick and simple preparation/amplification
of DNA samples, combined with fast DNA sequencing,
is of great interest to the forensic genetics
community. In this study the QuanTyper™-48
instrument has been used to speed up the
amplification steps involved in Sanger DNA
sequencing.
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Application
Note #3
Discrimination of base-pair
neutral single nucleotide polymorphism (SNP)
by highresolution melting on QuanTyper™-48
High-resolution melting
(HRM) analysis has recently emerged as a
simple, rapid and cost-effective method
for mutation screening and genotyping of
single nucleotide polymorphisms. In this
study, we show that the most subtle and
hard to detect genetic variation, an A/T
conversion, is possible to genotype by HRM
on QuanTyper-48.
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