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test results are critical in certain clinical
situations. For example, during surgery
(in particular during organ transplantation
operations) or when diagnosing infections
like meningitis and sepsis.
Rapid test results are also important in
biopreparedness for detection
of biohazardous agents
like the anthrax bacteria. The AlphaHelix
QuanTyper platform supports these applications,
producing better results in a fraction of
the time compared to conventional thermal
cyclers (see Fast
PCR). You will also get more done during
your working day. Imagine carrying
out four separate 40 cycle qPCR runs in
one hour!
Inhibiting substances,
in an often degraded sample, reduce sensitivity
and may prohibit DNA-identification in areas
like veterinary diagnostics, forensic
DNA analysis, food pathogen detection and
biosafety analyses. Regardless
of extraction method, inhibitor concentration
can be cut in at least half using AlphaHelix
instruments (see Application
note 1).
AlphaHelix’ platform for
diagnostic qPCR and cycle sequencing enables
more than just detection and quantification.
Viruses like avian flu
virus are “moving targets” for probe-based
testing. Therefore, DNA-sequencing is preferred.
QuanTyper™-48 runs cycle sequencing reactions
as rapidly as qPCR. And, when linked to
equipment for rapid capillary electrophoresis,
genotyping using Sanger sequencing, for
example, is possible within two hours (see
Application
note 2).
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Sequence data represents
the ‘gold standard’ not only for virus
detection but also in bacterial analysis.
The emerging area of multi
locus sequence typing (MLST)
is developing rapidly and is likely
to become the standard for bacterial
identification before long. (Read
more about MLST at www.mlst.net.)
High Resolution Melting
(HRM) analysis has recently evolved
as a powerful way to rapidly genotype
DNA, by discriminating between PCR
products differing by one or more
nucleotides (see Application
note 3). As such, HRM represents
a unique tool to get preliminary sequence
data in just a few minutes without
opening the tube or consuming any
of the material.
See also AlphaHelix
Reagent Cartridge |
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