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Overcoming PCR Inhibition    
 
Use of large-volume superconvective real-time PCR to overcome inhibitor induced amplification failure in bovine faeces samples.
     
25 µl
   
 
     
 

Positive control, 25 µl

 

10% DNA prep from faeces, 25 µl

  20% DNA prep from faeces, 25 µl
     
50 µl
   
 
     
 

Positive control, 50 µl

 

5% DNA prep from faeces, 50 µl

  10% DNA prep from faeces, 50 µl
     
100 µl
   
 
     
 

Positive control, 100 µl

 

2.5% DNA prep from faeces, 100 µl

  5% DNA prep from faeces, 100 µl
     
200 µl
   
 
     
 

Positive control, 200 µl

 

1.25% DNA prep from faeces, 200 µl

  2.5% DNA prep from faeces, 200 µl
     
For details about this proof-of-principle study see Application note 1.
     

 
 
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