Rapid PCR is both specific and sensitive. QuanTyper™-48 is able to detect a single gene copy in less than 14 minutes. Slower ramping in a conventional block cycler is less sensitive and produces more non-specific amplification.

The figures below show the result of a parallel comparison of amplification efficacy for identical 20 µl samples run on a QuanTyper-48 vs. ABI 7500. PCR reaction mixes contained SYBR Green I for real-time detection. Melting point and agarose gel analysis was used to discriminate between specific and non-specific amplification.

The PCR template was human DNA prepared from blood and 10-fold serially diluted from 50 ng down to 5 pg (5 pg roughly corresponds to one human haploid copy of DNA). Amplicon length is 115 bp.