Accurate Temperature Control


While all other PCR and qPCR instruments estimate sample temperature by measuring the block temperature or, in the case of LightCycler 2.0 and Rotor-Gene 6000, the air temperature, QuanTyper-48 continuously monitor sample temperature in real-time (throughout the run) using in-tube temperature measurement and temperature sensitive resistors. Figures 1-3 below highlight the benefits of in-tube temperature measurement.

 

The problem when estimating sample temperature from block or air temperature is that, since temperature ramping is slower the larger the reaction volume is, it is often impossible to get a PCR reaction and temperature profile to work equally well for different reaction volumes.

 

AlphaHelix's in-tube temperature measurement technology safely avoids this problem and ensures that the result will be the same, regardless of the total reaction volume.

 


 

See our publications and application notes on aAmp, SuperConvection and AmpXpress here

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