Speed
Rob™ has two modes for doing QPCR set-up. Traditional mode uses one tip for every sample replicate and OneDip™ mode uses the same tip for all replicates doing serial dispensing. The example below is based on a QPCR set-up with three replicates and pre-made master mix.
One tip per replicate
96-plate: 27 minutes (18 µl mix + 2 µl sample)
384-plate: 95 minutes (5 µl mix + 2 µl sample)
OneDip™
96-plate: 13 minutes (18 µl mix + 2 µl sample)
384-plate: 45 minutes (5 µl mix + 2 µl sample)
Rob™ handles a 384 set-up with acceptable speed but with low investment cost.