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Did you think all
thermal cyclers are the same?
Think Again. Think AmpXpress. |
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20
µl reaction volume. Phusion Flash Master Mix.
Amplicon: 596 bp. GC: content 64%.
Temperature profile: 98°C for 20 sec, followed
by 40 cycles of:
98°C for 0 sec; 63°C for 10 sec; 72°C for
3 sec.
Total run time: AmpXpress 22 minutes, Piko
33 minutes. |
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